Hydrochloric acid denaturation of colorectal tumour tissue infiltrated with bromodeoxyuridine

Thermal denaturation of DNA from bromodeoxyuridine substituted cells

The thermal denaturation of DNA from cell lines extensively substituted with bromodeoxyuridine has been examined spectrophotometrically over a wide range in ionic strength and by thermal elution from hydroxyapatite columns. BrdU substitution stabliizes DNA at all ionic strengths between 7.5 mM and 1350 mM potassium ion concentration, although a plot of log ionic strength vs Tm deviates from lin...

The Combination of Edestin with Hydrochloric Acid

Electromotive force measurements of cells without liquid junction, of the type Ag, AgCl, HCl + protein, H(2), lead to the conclusion that 1 gm. of edestin (or, more probably, edestan) combines with a maximum of 13.4 x 10(-4) equivalents of H(+) and 3.9 x 10(-4) equivalents of Cl(-), when the protein is dissolved in 0.1 M HCl.

The Combination of Gelatin with Hydrochloric Acid

1. Cooper's gelatin purified according to Northrop and Kunitz exhibited a minimum of osmotic pressure and a maximum of opacity at pH 5.05 +/-0.05. The pH of solutions of this gelatin in water was also close to this value. It is inferred that such gelatin is isoelectric at this pH and not at pH 4.70. 2. Hydrogen electrode measurements with KCl-agar junctions were made with concentrated solutions...

Metabolic alkalosis treated with intravenous hydrochloric acid.

Evaluation of MLL2 (KMT2B) Gene Expression in Colorectal Cancer Tissue Compared with Adjacent Tumor Free Tissue

Background and Aims: An impairment of expression in MLL2 gene involved in histone methylation induces alteration in the methylation patterns at enhancers and causes several cancers including colon cancer. In this study, MLL2 expression was evaluated in North West of Iran. Materials and Methods: To evaluate the MLL2 expression in patients with colon cancer, 25 tumor samples and 25 samples of tu...